Journal of Clinical Dentistry
Journal Details
Volume
39
Issue
1
Year
2026
ISSN
0895-8831
Journal of Clinical Dentistry
Indexed in PubMed
Pages 39 – 53
Randomized Controlled, Clinical Blinded Study to Evaluate the Main Cause of Periodontal Disease, the Predominant Presence of Bacteria in Percentage and the Total Bacterial Load Before and After Non-Surgical Therapy
Authors
Giuseppe Grech1*
Affiliations
1 Aprilia LT, II level Master of Periodontology Faculty of Medicine and Dentistry, Sapienza University of Rome, 12/2014
Abstract
Goals: Evaluate the root cause of periodontal disease, the predominance of bacteria and the total bacterial load before and after.

Materials and Methods: Microbiological Tests: it consists in taking the crevicular fluid colonized by bacteria and containing epithelial cells of the person being examined, using a sterile paper cone with a diameter of 60/80 in the periodontal or peri-implant pocket for at least 30 seconds (so that it is soaked in liquid crevicular possibly without blood); it is placed inside the test tube; the procedure is repeated to obtain from a minimum of two to a maximum of four paper cones. The test was performed on 420 patients aged 30-60 years, 236 women, 184 men, 4-11mm pockets moderate-severe chronic periodontitis, for a total of 498 samples, in 78 patients a second sampling was carried out after treatment.

Results: The study highlighted that the main cause of periodontal disease are parafunctions (Bruxism, clenching, bad habits, atypical swallowing, mouth breathing), dental misalignment, pre-contacts and incongruous prosthetic products. Therefore, as "direct and triggering" local etiological factors and no longer "indirect and predisposing" as has always been claimed. In the background by bacteria.

Keywords: Periodontal disease, inflammation, plaque, tartar, bacteria, microbiological analysis, parafunctions.
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📚 References
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❝ Cite this Paper
Giuseppe Grech, Randomized Controlled, Clinical Blinded Study to Evaluate the Main Cause of Periodontal Disease, the Predominant Presence of Bacteria in Percentage and the Total Bacterial Load Before and After Non-Surgical Therapy , Journal of Clinical Dentistry, Volume 39, Year 2026, PP. 39–53.

Randomized Controlled, Clinical Blinded Study to Evaluate the Main Cause of Periodontal Disease, the Predominant Presence of Bacteria in Percentage and the Total Bacterial Load Before and After Non-Surgical Therapy
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© 2026 Giuseppe Grech et al.
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